DBNascent | Samples

SRR7458429 Sample ID 2483

**Data sources**
Paper identifier	Tome2018single
SRP accession	SRP151707
GEO accession	GSE116472
Samples	SRR7458429

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-cap variant
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	39 Mb
Stranded gene read counts	TXT file	922 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	MEF
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat shock (42 Celsius)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	CoPRO - paired end PRO-cap; Capped RNA selection; with PCR amplification
Analysis notes	Many pretty dirty, high adapter contamination

**Sample metrics**
Sample QC score	4
Sample NRO score	5
Raw read length	76
Read depth after trimming	37,395,382
Duplication proportion	0.88185
Proportion reads mapped	0.9016
Exon/intron ratio	12.7599
Unique read proportion	0.25034

**Bidirectionals**
Total # Tfit bidirectionals	31336
# Promoter Tfit bidirectionals	10477
# Intronic Tfit bidirectionals	12098
# Intergenic Tfit bidirectionals	6834
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	True
Part of dREG master merge?	True