DBNascent | Samples

SRR7518313 Sample ID 2867

**Data sources**
Paper identifier	Zhu2018rna
SRP accession	SRP153346
GEO accession	GSE117014
Samples	SRR7518313

**Technical details**
QC score	4
Replicate	1
Protocol	NET-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 forward
Reverse complemented?	True
Mapped strandedness	read1 forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	12 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	A. thaliana
Sample type	tissue
Cell type	seedling
Clone/Individual
Strain	Col-0
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Antibody: 8WG16; unphosphorylated Pol II CTD antibody
Analysis notes	did fastq-dump manually, force trimmed first 10 bp and bp 100+ prior to running pipeline, ran pipeline on trimmed fastqs (w/normal bbduk trimming); ran tech reps on pre-trimmed fastqs directly

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	91
Read depth after trimming	20,220,749
Duplication proportion	0.72078
Proportion reads mapped	0.2471
Exon/intron ratio	1.96575
Unique read proportion	0.21541

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False