DBNascent | Samples

SRR7519714 Sample ID 2580

**Data sources**
Paper identifier	Viiri2019extensive
SRP accession	SRP153391
GEO accession	GSE117086
Samples	SRR7519714

**Technical details**
QC score	2
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	69 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	987 Kb
dREG bidirectional regions	BED file	357 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	iPSC
Clone/Individual	UTA.10100.EURCC
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	hepatocyte differentiation protocol 2	None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Treatment M1 denotes HLCs differentiated with method 1 (Si-Tayeb et al. 2010) and M2 uses method 2 (Hay et al. 2008), detailed further in supplementary methods
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	1
Raw read length	51
Read depth after trimming	22,098,398
Duplication proportion	0.55269
Proportion reads mapped	0.8909
Exon/intron ratio	1.78319
Unique read proportion	0.61732

**Bidirectionals**
Total # Tfit bidirectionals	11276
# Promoter Tfit bidirectionals	3522
# Intronic Tfit bidirectionals	4815
# Intergenic Tfit bidirectionals	2322
Total # dREG bidirectionals	8298
# Promoter dREG bidirectionals	3852
# Intronic dREG bidirectionals	3242
# Intergenic dREG bidirectionals	1423
Part of Tfit master merge?	True
Part of dREG master merge?	True