DBNascent | Samples

SRR7550989 Sample ID 1911

**Data sources**
Paper identifier	Nair2019phase
SRP accession	SRP154849
GEO accession	GSE117493
Samples	SRR7550989

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	93 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	1,6-hexanediol (8 percent)	-35 min	-30 min	5 min
treatment	TNF-alpha (100 nM)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes	Methods unclear in spots - treatments may not be accurate
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	100
Read depth after trimming	51,759,246
Duplication proportion	0.62945
Proportion reads mapped	0.7158
Exon/intron ratio	3.55676
Unique read proportion	0.46393

**Bidirectionals**
Total # Tfit bidirectionals	45547
# Promoter Tfit bidirectionals	14217
# Intronic Tfit bidirectionals	20563
# Intergenic Tfit bidirectionals	8405
Total # dREG bidirectionals	24602
# Promoter dREG bidirectionals	8128
# Intronic dREG bidirectionals	10695
# Intergenic dREG bidirectionals	5746
Part of Tfit master merge?	True
Part of dREG master merge?	True