DBNascent | Samples

SRR7550996 Sample ID 1918

**Data sources**
Paper identifier	Nair2019phase
SRP accession	SRP154850
GEO accession	GSE117494
Samples	SRR7550996

**Technical details**
QC score	3
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	65 Mb
Stranded gene read counts	TXT file	911 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	619 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	RAW264.7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	1,6-hexanediol (8.5 percent)	-35 min	-5 min	30 min
treatment	Kdo2-Lipid A (100 nM)	-5 min	0 min	5 min

**Extended notes**
Pipeline info and software versions
Sample notes	Methods unclear in spots - treatments may not be accurate
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	100
Read depth after trimming	49,118,375
Duplication proportion	0.70704
Proportion reads mapped	0.6909
Exon/intron ratio	2.77494
Unique read proportion	0.36419

**Bidirectionals**
Total # Tfit bidirectionals	23622
# Promoter Tfit bidirectionals	6277
# Intronic Tfit bidirectionals	9371
# Intergenic Tfit bidirectionals	4879
Total # dREG bidirectionals	14293
# Promoter dREG bidirectionals	4530
# Intronic dREG bidirectionals	4648
# Intergenic dREG bidirectionals	3856
Part of Tfit master merge?	True
Part of dREG master merge?	True