DBNascent | Samples

SRR7722985 Sample ID 602

**Data sources**
Paper identifier	Delgado-benito2018chromatin
SRP accession	SRP158419
GEO accession	GSE118794
Samples	SRR7722985

**Technical details**
QC score	3
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	150 Mb
Stranded gene read counts	TXT file	919 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	809 Kb
dREG bidirectional regions	BED file	607 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	B cell
Clone/Individual
Strain
Genotype	Zmynd8 fl/fl; Cd19-Cre
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	interleukin-4 (5 ng/mL)	-1 day	0 day	1 day
treatment	lipopolysaccharide (25 microg/mL)	-1 day	0 day	1 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	50
Read depth after trimming	157,832,147
Duplication proportion	0.77279
Proportion reads mapped	0.6979
Exon/intron ratio	3.68908
Unique read proportion	0.29416

**Bidirectionals**
Total # Tfit bidirectionals	9490
# Promoter Tfit bidirectionals	4054
# Intronic Tfit bidirectionals	3244
# Intergenic Tfit bidirectionals	595
Total # dREG bidirectionals	13995
# Promoter dREG bidirectionals	4490
# Intronic dREG bidirectionals	4491
# Intergenic dREG bidirectionals	3308
Part of Tfit master merge?	True
Part of dREG master merge?	True