DBNascent | Samples

SRR7879129 Sample ID 2271

**Data sources**
Paper identifier	Sheridan2019widespread
SRP accession	SRP162163
GEO accession	GSE120201
Samples	SRR7879129

**Technical details**
QC score	3
Replicate	2
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	62 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	516 Kb
dREG bidirectional regions	BED file	111 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype	dox-inducible TFIIS D282A, E283A (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	bromouridine (2 mM)	-30 min	0 min	30 min
treatment	dimethyloxalylglycine (2 mM)	-16 hr	0 hr	16 hr
vehicle	water	-40 hr	-16 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq; not clear if BrU was added after DMOG/dox washouts or during last 30 min
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	151
Read depth after trimming	13,027,136
Duplication proportion	0.19022
Proportion reads mapped	0.9639
Exon/intron ratio	1.48907
Unique read proportion	0.8129

**Bidirectionals**
Total # Tfit bidirectionals	5839
# Promoter Tfit bidirectionals	1695
# Intronic Tfit bidirectionals	3091
# Intergenic Tfit bidirectionals	427
Total # dREG bidirectionals	2564
# Promoter dREG bidirectionals	1192
# Intronic dREG bidirectionals	1050
# Intergenic dREG bidirectionals	238
Part of Tfit master merge?	True
Part of dREG master merge?	True