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SRR7879130 Sample ID 2272
| Paper identifier | Sheridan2019widespread |
| SRP accession | SRP162163 |
| GEO accession | GSE120201 |
| Samples | SRR7879130 |
| QC score | 4 |
| Replicate | 1 |
| Protocol | Bru-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | control |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 43 Mb |
| Stranded gene read counts | TXT file | 1 Mb |
| Unstranded bidirectional read counts | TXT file | 21 Mb |
| Tfit bidirectional regions | BED file | 465 Kb |
| dREG bidirectional regions | BED file | 88 Kb |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T Flp-In |
| Clone/Individual | |
| Strain | |
| Genotype | dox-inducible TFIIS D282A, E283A (Flp knock-in) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | bromouridine (2 mM) | -30 min | 0 min | 30 min |
| vehicle | water | -1 day | 0 day | 1 day |
| Pipeline info and software versions | |
| Sample notes | BrU-seq; not clear if BrU was added after DMOG/dox washouts or during last 30 min |
| Analysis notes |
| Sample QC score | 4 |
| Sample NRO score | 1 |
| Raw read length | 151 |
| Read depth after trimming | 9,744,821 |
| Duplication proportion | 0.37657 |
| Proportion reads mapped | 0.9591 |
| Exon/intron ratio | 1.98837 |
| Unique read proportion | 0.60997 |
| Total # Tfit bidirectionals | 5256 |
| # Promoter Tfit bidirectionals | 1863 |
| # Intronic Tfit bidirectionals | 2494 |
| # Intergenic Tfit bidirectionals | 278 |
| Total # dREG bidirectionals | 2027 |
| # Promoter dREG bidirectionals | 1125 |
| # Intronic dREG bidirectionals | 631 |
| # Intergenic dREG bidirectionals | 238 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |