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SRR7879131 Sample ID 2273
| Paper identifier | Sheridan2019widespread |
| SRP accession | SRP162163 |
| GEO accession | GSE120201 |
| Samples | SRR7879131 |
| QC score | 1 |
| Replicate | 1 |
| Protocol | Bru-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | False |
| Control/Experimental | control |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T Flp-In |
| Clone/Individual | |
| Strain | |
| Genotype | dox-inducible TFIIS D282A, E283A (Flp knock-in) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | bromouridine (2 mM) | -30 min | 0 min | 30 min |
| vehicle | water | -1 day | 0 day | 1 day |
| Pipeline info and software versions | |
| Sample notes | BrU-seq; not clear if BrU was added after DMOG/dox washouts or during last 30 min |
| Analysis notes |
| Sample QC score | 1 |
| Sample NRO score | 1 |
| Raw read length | 151 |
| Read depth after trimming | 26,708,147 |
| Duplication proportion | 0.28871 |
| Proportion reads mapped | 0.9634 |
| Exon/intron ratio | 1.95136 |
| Unique read proportion | 0.77544 |
| Total # Tfit bidirectionals | 9121 |
| # Promoter Tfit bidirectionals | 3552 |
| # Intronic Tfit bidirectionals | 4093 |
| # Intergenic Tfit bidirectionals | 663 |
| Total # dREG bidirectionals | 7004 |
| # Promoter dREG bidirectionals | 3290 |
| # Intronic dREG bidirectionals | 2500 |
| # Intergenic dREG bidirectionals | 560 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |