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SRR7879135 Sample ID 2277
| Paper identifier | Sheridan2019widespread |
| SRP accession | SRP162163 |
| GEO accession | GSE120201 |
| Samples | SRR7879135 |
| QC score | 3 |
| Replicate | 1 |
| Protocol | Bru-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T Flp-In |
| Clone/Individual | |
| Strain | |
| Genotype | dox-inducible TFIIS D282A, E283A (Flp knock-in) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | DRB (100 microM) | -190 min | -10 min | 3 hr |
| treatment | bromouridine (2 mM) | -10 min | 0 min | 10 min |
| vehicle | water | -1630 min | -190 min | 1 day |
| Pipeline info and software versions | |
| Sample notes | BrU-seq |
| Analysis notes |
| Sample QC score | 3 |
| Sample NRO score | 1 |
| Raw read length | 51 |
| Read depth after trimming | 10,768,145 |
| Duplication proportion | 0.34398 |
| Proportion reads mapped | 0.9384 |
| Exon/intron ratio | 2.36036 |
| Unique read proportion | 0.65322 |
| Total # Tfit bidirectionals | 18095 |
| # Promoter Tfit bidirectionals | 6941 |
| # Intronic Tfit bidirectionals | 7805 |
| # Intergenic Tfit bidirectionals | 1334 |
| Total # dREG bidirectionals | 8156 |
| # Promoter dREG bidirectionals | 3802 |
| # Intronic dREG bidirectionals | 2909 |
| # Intergenic dREG bidirectionals | 686 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |