DBNascent | Samples

SRR7879135 Sample ID 2277

**Data sources**
Paper identifier	Sheridan2019widespread
SRP accession	SRP162163
GEO accession	GSE120201
Samples	SRR7879135

**Technical details**
QC score	3
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	49 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	354 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype	dox-inducible TFIIS D282A, E283A (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-190 min	-10 min	3 hr
treatment	bromouridine (2 mM)	-10 min	0 min	10 min
vehicle	water	-1630 min	-190 min	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	51
Read depth after trimming	10,768,145
Duplication proportion	0.34398
Proportion reads mapped	0.9384
Exon/intron ratio	2.36036
Unique read proportion	0.65322

**Bidirectionals**
Total # Tfit bidirectionals	18095
# Promoter Tfit bidirectionals	6941
# Intronic Tfit bidirectionals	7805
# Intergenic Tfit bidirectionals	1334
Total # dREG bidirectionals	8156
# Promoter dREG bidirectionals	3802
# Intronic dREG bidirectionals	2909
# Intergenic dREG bidirectionals	686
Part of Tfit master merge?	True
Part of dREG master merge?	True