DBNascent | Samples

SRR7879136 Sample ID 2278

**Data sources**
Paper identifier	Sheridan2019widespread
SRP accession	SRP162163
GEO accession	GSE120201
Samples	SRR7879136

**Technical details**
QC score	4
Replicate	1
Protocol	Bru-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	47 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	639 Kb
dREG bidirectional regions	BED file	195 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T Flp-In
Clone/Individual
Strain
Genotype	dox-inducible TFIIS D282A, E283A (Flp knock-in)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	DRB (100 microM)	-200 min	-20 min	3 hr
treatment	bromouridine (2 mM)	-20 min	0 min	20 min
vehicle	water	-1640 min	-200 min	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	BrU-seq
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	51
Read depth after trimming	9,368,207
Duplication proportion	0.27259
Proportion reads mapped	0.9399
Exon/intron ratio	2.18689
Unique read proportion	0.71175

**Bidirectionals**
Total # Tfit bidirectionals	7312
# Promoter Tfit bidirectionals	2597
# Intronic Tfit bidirectionals	3307
# Intergenic Tfit bidirectionals	545
Total # dREG bidirectionals	4498
# Promoter dREG bidirectionals	1917
# Intronic dREG bidirectionals	1797
# Intergenic dREG bidirectionals	432
Part of Tfit master merge?	True
Part of dREG master merge?	True