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SRR7879137 Sample ID 2279
| Paper identifier | Sheridan2019widespread |
| SRP accession | SRP162163 |
| GEO accession | GSE120201 |
| Samples | SRR7879137 |
| QC score | 4 |
| Replicate | 1 |
| Protocol | Bru-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T Flp-In |
| Clone/Individual | |
| Strain | |
| Genotype | dox-inducible TFIIS D282A, E283A (Flp knock-in) |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | DRB (100 microM) | -185 min | -5 min | 3 hr |
| treatment | bromouridine (2 mM) | -5 min | 0 min | 5 min |
| vehicle | water | -1625 min | -185 min | 1 day |
| Pipeline info and software versions | |
| Sample notes | BrU-seq |
| Analysis notes |
| Sample QC score | 4 |
| Sample NRO score | 3 |
| Raw read length | 51 |
| Read depth after trimming | 8,348,245 |
| Duplication proportion | 0.49902 |
| Proportion reads mapped | 0.9482 |
| Exon/intron ratio | 5.38158 |
| Unique read proportion | 0.47849 |
| Total # Tfit bidirectionals | 22596 |
| # Promoter Tfit bidirectionals | 8519 |
| # Intronic Tfit bidirectionals | 10176 |
| # Intergenic Tfit bidirectionals | 918 |
| Total # dREG bidirectionals | 7433 |
| # Promoter dREG bidirectionals | 3677 |
| # Intronic dREG bidirectionals | 2396 |
| # Intergenic dREG bidirectionals | 545 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |