DBNascent | Samples

SRR797533 Sample ID 1071

**Data sources**
Paper identifier	Ji2013sr
SRP accession	SRP020006
GEO accession	GSE45517
Samples	SRR797533

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	18 Mb
Stranded gene read counts	TXT file	905 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	625 Kb
dREG bidirectional regions	BED file	262 Kb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	primary cell
Cell type	MEF
Clone/Individual
Strain
Genotype	Srsf1 -/-; dox-repressible Srsf1 exogenous (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Poor quality libraries; Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	34
Read depth after trimming	20,936,132
Duplication proportion	0.82492
Proportion reads mapped	0.3689
Exon/intron ratio	2.28826
Unique read proportion	0.15752

**Bidirectionals**
Total # Tfit bidirectionals	7374
# Promoter Tfit bidirectionals	3812
# Intronic Tfit bidirectionals	2014
# Intergenic Tfit bidirectionals	728
Total # dREG bidirectionals	6091
# Promoter dREG bidirectionals	4131
# Intronic dREG bidirectionals	920
# Intergenic dREG bidirectionals	505
Part of Tfit master merge?	True
Part of dREG master merge?	True