DBNascent | Samples

SRR8033058 Sample ID 697

**Data sources**
Paper identifier	Esousa2019kinetics
SRP accession	SRP165220
GEO accession	GSE121144
Samples	SRR8033058

**Technical details**
QC score	1
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	125 Mb
Stranded gene read counts	TXT file	924 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual	TX1072
Strain	C57BL/6 x CAST/EiJ
Genotype	dox-inducible Xist endogenous allele
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-24 hr	0 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	101
Read depth after trimming	41,189,366
Duplication proportion	0.42364
Proportion reads mapped	0.7938
Exon/intron ratio	3.27983
Unique read proportion	0.68572

**Bidirectionals**
Total # Tfit bidirectionals	52207
# Promoter Tfit bidirectionals	11810
# Intronic Tfit bidirectionals	17109
# Intergenic Tfit bidirectionals	18707
Total # dREG bidirectionals	36825
# Promoter dREG bidirectionals	8108
# Intronic dREG bidirectionals	11461
# Intergenic dREG bidirectionals	14328
Part of Tfit master merge?	True
Part of dREG master merge?	True