DBNascent | Samples

SRR8234177 Sample ID 2247

**Data sources**
Paper identifier	Sendinc2019pcif1
SRP accession	SRP170033
GEO accession	GSE122802
Samples	SRR8234177

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	139 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MEL624
Clone/Individual	sgRNA Pcif1 clone 2
Strain
Genotype	Pcif1 -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	42
Read depth after trimming	28,433,680
Duplication proportion	0.27837
Proportion reads mapped	0.7314
Exon/intron ratio	3.81223
Unique read proportion	0.68291

**Bidirectionals**
Total # Tfit bidirectionals	39739
# Promoter Tfit bidirectionals	10740
# Intronic Tfit bidirectionals	17909
# Intergenic Tfit bidirectionals	11049
Total # dREG bidirectionals	27692
# Promoter dREG bidirectionals	8385
# Intronic dREG bidirectionals	11919
# Intergenic dREG bidirectionals	7217
Part of Tfit master merge?	True
Part of dREG master merge?	True