DBNascent | Samples

SRR828696 Sample ID 872

**Data sources**
Paper identifier	Galbraith2013hif1a
SRP accession	SRP013319
GEO accession	GSE38140
Samples	SRR828696

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	True
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	248 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow; originally labeled and processed as tech reps, but clearly says in the paper that they're biological replicates

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	50
Read depth after trimming	198,849,055
Duplication proportion	0.83445
Proportion reads mapped	0.8927
Exon/intron ratio	2.57436
Unique read proportion	0.65601

**Bidirectionals**
Total # Tfit bidirectionals	43099
# Promoter Tfit bidirectionals	16507
# Intronic Tfit bidirectionals	15695
# Intergenic Tfit bidirectionals	9797
Total # dREG bidirectionals	42349
# Promoter dREG bidirectionals	8378
# Intronic dREG bidirectionals	19405
# Intergenic dREG bidirectionals	12934
Part of Tfit master merge?	True
Part of dREG master merge?	True