DBNascent | Samples

SRR8364245 Sample ID 3

**Data sources**
Paper identifier	Aeby2020decapping
SRP accession	SRP174237
GEO accession	GSE124289
Samples	SRR8364245

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	122 Mb
Stranded gene read counts	TXT file	927 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	6 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	cell line
Cell type	ESC
Clone/Individual
Strain	C57BL/6J x CAST/EiJ
Genotype	Tsix +/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	embryoid body differentiation protocol	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes	Differentiation induced by leaving out 50 U/mL ESGRO leukaemia inhibitory factor (LIF); Library prep cites Kwak2013 and Mahat2016
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	3
Raw read length	51
Read depth after trimming	32,706,367
Duplication proportion	0.3562
Proportion reads mapped	0.801
Exon/intron ratio	5.88963
Unique read proportion	0.61188

**Bidirectionals**
Total # Tfit bidirectionals	69002
# Promoter Tfit bidirectionals	12968
# Intronic Tfit bidirectionals	25467
# Intergenic Tfit bidirectionals	25777
Total # dREG bidirectionals	50841
# Promoter dREG bidirectionals	9265
# Intronic dREG bidirectionals	18556
# Intergenic dREG bidirectionals	18488
Part of Tfit master merge?	True
Part of dREG master merge?	True