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SRR8364247 Sample ID 5
| Paper identifier | Aeby2020decapping |
| SRP accession | SRP174237 |
| GEO accession | GSE124289 |
| Samples | SRR8364247 |
| QC score | 3 |
| Replicate | 1 |
| Protocol | PRO-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | paired |
| Raw strandedness | read1 reverse |
| Reverse complemented? | False |
| Mapped strandedness | read1 reverse |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 68 Mb |
| Stranded gene read counts | TXT file | 926 Kb |
| Unstranded bidirectional read counts | TXT file | 17 Mb |
| Tfit bidirectional regions | BED file | 2 Mb |
| dREG bidirectional regions | BED file | 949 Kb |
| All data files | ZIP archive | |
| Organism | M. musculus |
| Sample type | cell line |
| Cell type | ESC |
| Clone/Individual | |
| Strain | C57BL/6J x CAST/EiJ |
| Genotype | Tsix +/- |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | embryoid body differentiation protocol | -7 day | 0 day | 7 day |
| Pipeline info and software versions | |
| Sample notes | Differentiation induced by leaving out 50 U/mL ESGRO leukaemia inhibitory factor (LIF); Library prep cites Kwak2013 and Mahat2016 |
| Analysis notes |
| Sample QC score | 3 |
| Sample NRO score | 2 |
| Raw read length | 51 |
| Read depth after trimming | 33,185,131 |
| Duplication proportion | 0.73811 |
| Proportion reads mapped | 0.8427 |
| Exon/intron ratio | 4.67537 |
| Unique read proportion | 0.42163 |
| Total # Tfit bidirectionals | 22816 |
| # Promoter Tfit bidirectionals | 8007 |
| # Intronic Tfit bidirectionals | 8547 |
| # Intergenic Tfit bidirectionals | 4432 |
| Total # dREG bidirectionals | 21882 |
| # Promoter dREG bidirectionals | 8342 |
| # Intronic dREG bidirectionals | 7699 |
| # Intergenic dREG bidirectionals | 4046 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |