DBNascent | Samples

SRR8384738 Sample ID 1233

**Data sources**
Paper identifier	Korkmaz2019crispr
SRP accession	SRP175029
GEO accession	GSE124572
Samples	SRR8384738

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	99 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	834 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual	sgRNA 1830
Strain
Genotype	ER-alpha enhancer -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	sgRNA1830 targeting ERa binding site and serves here as another control
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	65
Read depth after trimming	26,656,967
Duplication proportion	0.37956
Proportion reads mapped	0.9949
Exon/intron ratio	3.19913
Unique read proportion	0.75117

**Bidirectionals**
Total # Tfit bidirectionals	32719
# Promoter Tfit bidirectionals	11574
# Intronic Tfit bidirectionals	14496
# Intergenic Tfit bidirectionals	5699
Total # dREG bidirectionals	19197
# Promoter dREG bidirectionals	8002
# Intronic dREG bidirectionals	7543
# Intergenic dREG bidirectionals	3388
Part of Tfit master merge?	True
Part of dREG master merge?	True