DBNascent | Samples

SRR8419431 Sample ID 1921

**Data sources**
Paper identifier	Nair2019phase
SRP accession	SRP176659
GEO accession	GSE124826
Samples	SRR8419431

**Technical details**
QC score	1
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	150 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	805 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-905 min	-5 min	15 hr
treatment	2,5-hexanediol (8.5 percent)	-5 min	0 min	5 min

**Extended notes**
Pipeline info and software versions
Sample notes	Methods unclear in spots - treatments may not be accurate
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	75
Read depth after trimming	45,670,350
Duplication proportion	0.30983
Proportion reads mapped	0.7863
Exon/intron ratio	3.34495
Unique read proportion	0.77419

**Bidirectionals**
Total # Tfit bidirectionals	29817
# Promoter Tfit bidirectionals	13551
# Intronic Tfit bidirectionals	11527
# Intergenic Tfit bidirectionals	4242
Total # dREG bidirectionals	18366
# Promoter dREG bidirectionals	7743
# Intronic dREG bidirectionals	6769
# Intergenic dREG bidirectionals	3424
Part of Tfit master merge?	True
Part of dREG master merge?	True