DBNascent | Samples

SRR8429052 Sample ID 2170

**Data sources**
Paper identifier	Sasse2019nascent
SRP accession	SRP178237
GEO accession	GSE124916
Samples	SRR8429052

**Technical details**
QC score	4
Replicate	1
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	137 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	BEAS-2B
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha (20 ng/mL)	-10 min	0 min	10 min
treatment	dexamethasone (100 nM)	-10 min	0 min	10 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	116,526,720
Duplication proportion	0.83484
Proportion reads mapped	0.906
Exon/intron ratio	2.09518
Unique read proportion	0.58421

**Bidirectionals**
Total # Tfit bidirectionals	31928
# Promoter Tfit bidirectionals	11561
# Intronic Tfit bidirectionals	12521
# Intergenic Tfit bidirectionals	7347
Total # dREG bidirectionals	24052
# Promoter dREG bidirectionals	7975
# Intronic dREG bidirectionals	10541
# Intergenic dREG bidirectionals	4975
Part of Tfit master merge?	True
Part of dREG master merge?	True