DBNascent | Samples

SRR8429053 Sample ID 2171

**Data sources**
Paper identifier	Sasse2019nascent
SRP accession	SRP178237
GEO accession	GSE124916
Samples	SRR8429053

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	36 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	908 Kb
dREG bidirectional regions	BED file	256 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	BEAS-2B
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha (20 ng/mL)	-10 min	0 min	10 min
treatment	dexamethasone (100 nM)	-10 min	0 min	10 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	59,057,507
Duplication proportion	0.93115
Proportion reads mapped	0.7815
Exon/intron ratio	2.98381
Unique read proportion	0.17049

**Bidirectionals**
Total # Tfit bidirectionals	10129
# Promoter Tfit bidirectionals	3174
# Intronic Tfit bidirectionals	4905
# Intergenic Tfit bidirectionals	1593
Total # dREG bidirectionals	5964
# Promoter dREG bidirectionals	3072
# Intronic dREG bidirectionals	2168
# Intergenic dREG bidirectionals	839
Part of Tfit master merge?	True
Part of dREG master merge?	True