DBNascent | Samples

SRR8429057 Sample ID 2175

**Data sources**
Paper identifier	Sasse2019nascent
SRP accession	SRP178237
GEO accession	GSE124916
Samples	SRR8429057

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	97 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	764 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	BEAS-2B
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha (20 ng/mL)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	108,866,146
Duplication proportion	0.88186
Proportion reads mapped	0.91
Exon/intron ratio	1.73521
Unique read proportion	0.48054

**Bidirectionals**
Total # Tfit bidirectionals	24204
# Promoter Tfit bidirectionals	8530
# Intronic Tfit bidirectionals	10373
# Intergenic Tfit bidirectionals	4675
Total # dREG bidirectionals	17615
# Promoter dREG bidirectionals	6595
# Intronic dREG bidirectionals	7393
# Intergenic dREG bidirectionals	3153
Part of Tfit master merge?	True
Part of dREG master merge?	True