DBNascent | Samples

SRR8429061 Sample ID 2179

**Data sources**
Paper identifier	Sasse2019nascent
SRP accession	SRP178237
GEO accession	GSE124916
Samples	SRR8429061

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	random primed
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	51 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	552 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	BEAS-2B
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	TNF-alpha (20 ng/mL)	-30 min	0 min	30 min
treatment	dexamethasone (100 nM)	-30 min	0 min	30 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	80,085,758
Duplication proportion	0.81565
Proportion reads mapped	0.3968
Exon/intron ratio	2.1405
Unique read proportion	0.37597

**Bidirectionals**
Total # Tfit bidirectionals	19142
# Promoter Tfit bidirectionals	6614
# Intronic Tfit bidirectionals	8437
# Intergenic Tfit bidirectionals	3331
Total # dREG bidirectionals	12793
# Promoter dREG bidirectionals	5497
# Intronic dREG bidirectionals	5111
# Intergenic dREG bidirectionals	2021
Part of Tfit master merge?	True
Part of dREG master merge?	True