DBNascent | Samples

SRR8452660 Sample ID 2023

**Data sources**
Paper identifier	Parida2019nucleotide
SRP accession	SRP141182
GEO accession	GSE113394
Samples	SRR8452660

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	38 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	785 Kb
dREG bidirectional regions	BED file	161 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	26,184,279
Duplication proportion	0.35968
Proportion reads mapped	0.2181
Exon/intron ratio	2.79758
Unique read proportion	0.45508

**Bidirectionals**
Total # Tfit bidirectionals	9057
# Promoter Tfit bidirectionals	5479
# Intronic Tfit bidirectionals	3308
# Intergenic Tfit bidirectionals	550
Total # dREG bidirectionals	3754
# Promoter dREG bidirectionals	3035
# Intronic dREG bidirectionals	798
# Intergenic dREG bidirectionals	277
Part of Tfit master merge?	True
Part of dREG master merge?	True