DBNascent | Samples

SRR8452661 Sample ID 2024

**Data sources**
Paper identifier	Parida2019nucleotide
SRP accession	SRP141182
GEO accession	GSE113394
Samples	SRR8452661

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	51 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	124 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
vehicle	DMSO (0.1%)	-1 hr	0 hr	1 hr
treatment	HCMV strain Towne varS	-5760 min	-5670 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	151
Read depth after trimming	23,890,943
Duplication proportion	0.25556
Proportion reads mapped	0.2509
Exon/intron ratio	2.22244
Unique read proportion	0.63948

**Bidirectionals**
Total # Tfit bidirectionals	12597
# Promoter Tfit bidirectionals	6588
# Intronic Tfit bidirectionals	4618
# Intergenic Tfit bidirectionals	1155
Total # dREG bidirectionals	2874
# Promoter dREG bidirectionals	2140
# Intronic dREG bidirectionals	646
# Intergenic dREG bidirectionals	255
Part of Tfit master merge?	True
Part of dREG master merge?	True