DBNascent | Samples

SRR8457216 Sample ID 2027

**Data sources**
Paper identifier	Parida2019nucleotide
SRP accession	SRP141182
GEO accession	GSE113394
Samples	SRR8457216

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	109 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	1 Mb
dREG bidirectional regions	BED file	727 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	primary cell
Cell type	foreskin fibroblast
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	flavopiridol (1 microM)	-1 hr	0 hr	1 hr
treatment	HCMV strain TB40/E (MOI 3)	-4 hr	0 hr	4 hr

**Extended notes**
Pipeline info and software versions
Sample notes	MOI around 3
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	151
Read depth after trimming	63,101,686
Duplication proportion	0.59254
Proportion reads mapped	0.7007
Exon/intron ratio	3.17704
Unique read proportion	0.38214

**Bidirectionals**
Total # Tfit bidirectionals	15175
# Promoter Tfit bidirectionals	7282
# Intronic Tfit bidirectionals	6455
# Intergenic Tfit bidirectionals	1363
Total # dREG bidirectionals	16905
# Promoter dREG bidirectionals	9161
# Intronic dREG bidirectionals	6633
# Intergenic dREG bidirectionals	1807
Part of Tfit master merge?	True
Part of dREG master merge?	True