DBNascent | Samples

SRR8478998 Sample ID 221

**Data sources**
Paper identifier	Beckedorff2020human
SRP accession	SRP181677
GEO accession	GSE125535
Samples	SRR8478998

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	393 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	5 Mb
dREG bidirectional regions	BED file	3 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype	dox-inducible shRNA control (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	133,314,870
Duplication proportion	0.49862
Proportion reads mapped	0.8862
Exon/intron ratio	3.09514
Unique read proportion	0.76776

**Bidirectionals**
Total # Tfit bidirectionals	61410
# Promoter Tfit bidirectionals	20095
# Intronic Tfit bidirectionals	21198
# Intergenic Tfit bidirectionals	20417
Total # dREG bidirectionals	59139
# Promoter dREG bidirectionals	8560
# Intronic dREG bidirectionals	27385
# Intergenic dREG bidirectionals	21002
Part of Tfit master merge?	True
Part of dREG master merge?	True