DBNascent | Samples

SRR8479006 Sample ID 229

**Data sources**
Paper identifier	Beckedorff2020human
SRP accession	SRP181677
GEO accession	GSE125535
Samples	SRR8479006

**Technical details**
QC score	1
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	433 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	7 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HeLa
Clone/Individual
Strain
Genotype	dox-inducible shRNA Ints11 endogenous (stable)
Construct	Ints11 E203Q exogenous

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-3 day	0 day	3 day

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	1
Sample NRO score	2
Raw read length	35
Read depth after trimming	162,802,108
Duplication proportion	0.4699
Proportion reads mapped	0.7576
Exon/intron ratio	3.03292
Unique read proportion	0.79762

**Bidirectionals**
Total # Tfit bidirectionals	75657
# Promoter Tfit bidirectionals	18880
# Intronic Tfit bidirectionals	28002
# Intergenic Tfit bidirectionals	26305
Total # dREG bidirectionals	55617
# Promoter dREG bidirectionals	8730
# Intronic dREG bidirectionals	25274
# Intergenic dREG bidirectionals	19532
Part of Tfit master merge?	True
Part of dREG master merge?	True