DBNascent | Samples

SRR8482947 Sample ID 213

**Data sources**
Paper identifier	Barucci2020small
SRP accession	SRP181890
GEO accession	GSE125597
Samples	SRR8482947

**Technical details**
QC score	4
Replicate	1
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	15 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	C. elegans
Sample type	organism
Cell type	whole worm
Clone/Individual	stage adult
Strain	N2
Genotype	prg-1 -/-
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	This method used Biotin-UTP though they claim to be using GRO-seq
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	4
Sample NRO score	3
Raw read length	35
Read depth after trimming	24,884,278
Duplication proportion	0.88567
Proportion reads mapped	0.5637
Exon/intron ratio	6.07638
Unique read proportion	0.16441

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False