SRR8482948 Sample ID 214

Data sources
Paper identifier Barucci2020small
SRP accession SRP181890
GEO accession GSE125597
Samples SRR8482948
Technical details
QC score 3
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness forward
Reverse complemented? False
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 41 Mb
Cellular details
Organism C. elegans
Sample type organism
Cell type whole worm
Clone/Individual stage adult
Strain N2
Genotype prg-1 -/-
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
no treatment None None None
Extended notes
Pipeline info and software versions
Sample notes This method used Biotin-UTP though they claim to be using GRO-seq
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 35
Read depth after trimming 24,618,802
Duplication proportion 0.71771
Proportion reads mapped 0.8367
Exon/intron ratio 3.25829
Unique read proportion 0.35514
Bidirectionals
Total # Tfit bidirectionals None
# Promoter Tfit bidirectionals None
# Intronic Tfit bidirectionals None
# Intergenic Tfit bidirectionals None
Total # dREG bidirectionals None
# Promoter dREG bidirectionals None
# Intronic dREG bidirectionals None
# Intergenic dREG bidirectionals None
Part of Tfit master merge? False
Part of dREG master merge? False