DBNascent | Samples

SRR8483106 Sample ID 2883

**Data sources**
Paper identifier	Zhu2019non
SRP accession	SRP181911
GEO accession	GSE125607
Samples	SRR8483106

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	136 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	762 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	shRNA control (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	54
Read depth after trimming	46,687,901
Duplication proportion	0.53267
Proportion reads mapped	0.8959
Exon/intron ratio	3.69714
Unique read proportion	0.66045

**Bidirectionals**
Total # Tfit bidirectionals	26991
# Promoter Tfit bidirectionals	10498
# Intronic Tfit bidirectionals	11985
# Intergenic Tfit bidirectionals	3408
Total # dREG bidirectionals	17479
# Promoter dREG bidirectionals	6658
# Intronic dREG bidirectionals	7009
# Intergenic dREG bidirectionals	2936
Part of Tfit master merge?	True
Part of dREG master merge?	True