DBNascent | Samples

SRR8483108 Sample ID 2885

**Data sources**
Paper identifier	Zhu2019non
SRP accession	SRP181911
GEO accession	GSE125607
Samples	SRR8483108

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	140 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	796 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual
Strain
Genotype	shRNA Tead4 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	17-beta-estradiol (100 nM)	-1 hr	0 hr	1 hr

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	54
Read depth after trimming	45,990,930
Duplication proportion	0.50628
Proportion reads mapped	0.8872
Exon/intron ratio	3.80345
Unique read proportion	0.67993

**Bidirectionals**
Total # Tfit bidirectionals	29947
# Promoter Tfit bidirectionals	10694
# Intronic Tfit bidirectionals	13800
# Intergenic Tfit bidirectionals	4063
Total # dREG bidirectionals	18268
# Promoter dREG bidirectionals	6937
# Intronic dREG bidirectionals	7328
# Intergenic dREG bidirectionals	3252
Part of Tfit master merge?	True
Part of dREG master merge?	True