SRR8608051 Sample ID 2180

Data sources
Paper identifier Sathyan2019improved
SRP accession SRP186571
GEO accession GSE126919
Samples SRR8608051
Technical details
QC score 3
Replicate 1
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 55 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 2 Mb
dREG bidirectional regions BED file 530 Kb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype Znf143 auxin-inducible degron; Tir1 knock-in
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
vehicle DMSO -3 hr 0 hr 3 hr
Extended notes
Pipeline info and software versions
Sample notes Tir1 insertion into Aavs1 locus
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 75
Read depth after trimming 16,722,870
Duplication proportion 0.21872
Proportion reads mapped 0.5471
Exon/intron ratio 3.73832
Unique read proportion 0.77731
Bidirectionals
Total # Tfit bidirectionals 19521
# Promoter Tfit bidirectionals 10149
# Intronic Tfit bidirectionals 7487
# Intergenic Tfit bidirectionals 2003
Total # dREG bidirectionals 12271
# Promoter dREG bidirectionals 8161
# Intronic dREG bidirectionals 3868
# Intergenic dREG bidirectionals 797
Part of Tfit master merge? True
Part of dREG master merge? True