SRR8608052 Sample ID 2181

Data sources
Paper identifier Sathyan2019improved
SRP accession SRP186571
GEO accession GSE126919
Samples SRR8608052
Technical details
QC score 3
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype Znf143 auxin-inducible degron; Tir1 knock-in
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
vehicle DMSO -3 hr 0 hr 3 hr
Extended notes
Pipeline info and software versions
Sample notes Tir1 insertion into Aavs1 locus
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 75
Read depth after trimming 19,695,769
Duplication proportion 0.22244
Proportion reads mapped 0.458
Exon/intron ratio 3.13231
Unique read proportion 0.77285
Bidirectionals
Total # Tfit bidirectionals 15843
# Promoter Tfit bidirectionals 8028
# Intronic Tfit bidirectionals 6030
# Intergenic Tfit bidirectionals 1713
Total # dREG bidirectionals 9637
# Promoter dREG bidirectionals 6855
# Intronic dREG bidirectionals 2721
# Intergenic dREG bidirectionals 556
Part of Tfit master merge? True
Part of dREG master merge? True