DBNascent | Samples

SRR8608054 Sample ID 2183

**Data sources**
Paper identifier	Sathyan2019improved
SRP accession	SRP186571
GEO accession	GSE126919
Samples	SRR8608054

**Technical details**
QC score	3
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	58 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	501 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	Znf143 auxin-inducible degron; Tir1 knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	auxin (500 microM)	-3 hr	0 hr	3 hr

**Extended notes**
Pipeline info and software versions
Sample notes	Tir1 insertion into Aavs1 locus
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	75
Read depth after trimming	17,234,709
Duplication proportion	0.19598
Proportion reads mapped	0.553
Exon/intron ratio	3.22989
Unique read proportion	0.80141

**Bidirectionals**
Total # Tfit bidirectionals	19430
# Promoter Tfit bidirectionals	10069
# Intronic Tfit bidirectionals	7711
# Intergenic Tfit bidirectionals	1956
Total # dREG bidirectionals	11626
# Promoter dREG bidirectionals	7747
# Intronic dREG bidirectionals	3713
# Intergenic dREG bidirectionals	799
Part of Tfit master merge?	True
Part of dREG master merge?	True