SRR8608054 Sample ID 2183

Data sources
Paper identifier Sathyan2019improved
SRP accession SRP186571
GEO accession GSE126919
Samples SRR8608054
Technical details
QC score 3
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end single
Raw strandedness reverse
Reverse complemented? True
Mapped strandedness forward
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype Znf143 auxin-inducible degron; Tir1 knock-in
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment auxin (500 microM) -3 hr 0 hr 3 hr
Extended notes
Pipeline info and software versions
Sample notes Tir1 insertion into Aavs1 locus
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 3
Sample NRO score 2
Raw read length 75
Read depth after trimming 17,234,709
Duplication proportion 0.19598
Proportion reads mapped 0.553
Exon/intron ratio 3.22989
Unique read proportion 0.80141
Bidirectionals
Total # Tfit bidirectionals 19430
# Promoter Tfit bidirectionals 10069
# Intronic Tfit bidirectionals 7711
# Intergenic Tfit bidirectionals 1956
Total # dREG bidirectionals 11626
# Promoter dREG bidirectionals 7747
# Intronic dREG bidirectionals 3713
# Intergenic dREG bidirectionals 799
Part of Tfit master merge? True
Part of dREG master merge? True