SRR8608068 Sample ID 2197

Data sources
Paper identifier Sathyan2019improved
SRP accession SRP186571
GEO accession GSE126919
Samples SRR8608068
Technical details
QC score 1
Replicate 2
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end paired
Raw strandedness read1 reverse
Reverse complemented? False
Mapped strandedness read1 reverse
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file 155 Mb
Stranded gene read counts TXT file 1 Mb
Unstranded bidirectional read counts TXT file 21 Mb
Tfit bidirectional regions BED file 2 Mb
dREG bidirectional regions BED file 708 Kb
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype Znf143 auxin-inducible degron; Tir1 knock-in; Arf16-PB1 knock-in
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment auxin (500 microM) -90 min 0 min 90 min
Extended notes
Pipeline info and software versions
Sample notes Tir1 insertion into Aavs1 locus, Znf143 tagged C-terminally with AID, PB1 domain of Arf16 expressed and cell sorted
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 1
Sample NRO score 2
Raw read length 35
Read depth after trimming 24,244,892
Duplication proportion 0.09818
Proportion reads mapped 0.6697
Exon/intron ratio 3.22307
Unique read proportion 0.84892
Bidirectionals
Total # Tfit bidirectionals 27875
# Promoter Tfit bidirectionals 14105
# Intronic Tfit bidirectionals 10435
# Intergenic Tfit bidirectionals 3730
Total # dREG bidirectionals 16342
# Promoter dREG bidirectionals 8217
# Intronic dREG bidirectionals 5654
# Intergenic dREG bidirectionals 2050
Part of Tfit master merge? True
Part of dREG master merge? True