DBNascent | Samples

SRR8608070 Sample ID 2199

**Data sources**
Paper identifier	Sathyan2019improved
SRP accession	SRP186571
GEO accession	GSE126919
Samples	SRR8608070

**Technical details**
QC score	3
Replicate	4
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	89 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	478 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	Znf143 auxin-inducible degron; Tir1 knock-in; Arf16-PB1 knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	auxin (500 microM)	-90 min	0 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes	Tir1 insertion into Aavs1 locus, Znf143 tagged C-terminally with AID, PB1 domain of Arf16 expressed and cell sorted
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	3
Sample NRO score	1
Raw read length	35
Read depth after trimming	14,091,152
Duplication proportion	0.08093
Proportion reads mapped	0.5836
Exon/intron ratio	2.96557
Unique read proportion	0.86085

**Bidirectionals**
Total # Tfit bidirectionals	22335
# Promoter Tfit bidirectionals	11395
# Intronic Tfit bidirectionals	8813
# Intergenic Tfit bidirectionals	2367
Total # dREG bidirectionals	11099
# Promoter dREG bidirectionals	7009
# Intronic dREG bidirectionals	3317
# Intergenic dREG bidirectionals	1035
Part of Tfit master merge?	True
Part of dREG master merge?	True