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SRR8608070 Sample ID 2199
| Paper identifier | Sathyan2019improved |
| SRP accession | SRP186571 |
| GEO accession | GSE126919 |
| Samples | SRR8608070 |
| QC score | 3 |
| Replicate | 4 |
| Protocol | PRO-seq |
| Library prep | ligation |
| Spike-in | None |
| Single/Paired end | paired |
| Raw strandedness | read1 reverse |
| Reverse complemented? | False |
| Mapped strandedness | read1 reverse |
| Timecourse | False |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | |
| Stranded gene read counts | TXT file | |
| Unstranded bidirectional read counts | TXT file | |
| Tfit bidirectional regions | BED file | |
| dREG bidirectional regions | BED file | |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | HEK293T |
| Clone/Individual | |
| Strain | |
| Genotype | Znf143 auxin-inducible degron; Tir1 knock-in; Arf16-PB1 knock-in |
| Construct |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | auxin (500 microM) | -90 min | 0 min | 90 min |
| Pipeline info and software versions | |
| Sample notes | Tir1 insertion into Aavs1 locus, Znf143 tagged C-terminally with AID, PB1 domain of Arf16 expressed and cell sorted |
| Analysis notes | Ran qc redo nextflow |
| Sample QC score | 3 |
| Sample NRO score | 1 |
| Raw read length | 35 |
| Read depth after trimming | 14,091,152 |
| Duplication proportion | 0.08093 |
| Proportion reads mapped | 0.5836 |
| Exon/intron ratio | 2.96557 |
| Unique read proportion | 0.86085 |
| Total # Tfit bidirectionals | 22335 |
| # Promoter Tfit bidirectionals | 11395 |
| # Intronic Tfit bidirectionals | 8813 |
| # Intergenic Tfit bidirectionals | 2367 |
| Total # dREG bidirectionals | 11099 |
| # Promoter dREG bidirectionals | 7009 |
| # Intronic dREG bidirectionals | 3317 |
| # Intergenic dREG bidirectionals | 1035 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |