SRR8608071 Sample ID 2200

Data sources
Paper identifier Sathyan2019improved
SRP accession SRP186571
GEO accession GSE126919
Samples SRR8608071
Technical details
QC score 2
Replicate 1
Protocol PRO-seq
Library prep ligation
Spike-in None
Single/Paired end paired
Raw strandedness read1 reverse
Reverse complemented? False
Mapped strandedness read1 reverse
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type HEK293T
Clone/Individual
Strain
Genotype Znf143 auxin-inducible degron; Tir1 knock-in
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment auxin (500 microM) -90 min 0 min 90 min
Extended notes
Pipeline info and software versions
Sample notes Tir1 insertion into Aavs1 locus, Znf143 tagged C-terminally with auxin-inducible degron (AID)
Analysis notes Ran qc redo nextflow
Sample metrics
Sample QC score 2
Sample NRO score 2
Raw read length 35
Read depth after trimming 21,764,884
Duplication proportion 0.09699
Proportion reads mapped 0.6632
Exon/intron ratio 3.17189
Unique read proportion 0.84867
Bidirectionals
Total # Tfit bidirectionals 27742
# Promoter Tfit bidirectionals 14099
# Intronic Tfit bidirectionals 10238
# Intergenic Tfit bidirectionals 3706
Total # dREG bidirectionals 15924
# Promoter dREG bidirectionals 8384
# Intronic dREG bidirectionals 5354
# Intergenic dREG bidirectionals 1995
Part of Tfit master merge? True
Part of dREG master merge? True