DBNascent | Samples

SRR8608073 Sample ID 2202

**Data sources**
Paper identifier	Sathyan2019improved
SRP accession	SRP186571
GEO accession	GSE126919
Samples	SRR8608073

**Technical details**
QC score	5
Replicate	3
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	33 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	598 Kb
dREG bidirectional regions	BED file	77 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HEK293T
Clone/Individual
Strain
Genotype	Znf143 auxin-inducible degron; Tir1 knock-in
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	auxin (500 microM)	-90 min	0 min	90 min

**Extended notes**
Pipeline info and software versions
Sample notes	Tir1 insertion into Aavs1 locus, Znf143 tagged C-terminally with auxin-inducible degron (AID)
Analysis notes	Ran qc redo nextflow

**Sample metrics**
Sample QC score	5
Sample NRO score	1
Raw read length	35
Read depth after trimming	3,246,944
Duplication proportion	0.06529
Proportion reads mapped	0.6801
Exon/intron ratio	2.95652
Unique read proportion	0.7641

**Bidirectionals**
Total # Tfit bidirectionals	6888
# Promoter Tfit bidirectionals	3951
# Intronic Tfit bidirectionals	2235
# Intergenic Tfit bidirectionals	563
Total # dREG bidirectionals	1782
# Promoter dREG bidirectionals	1196
# Intronic dREG bidirectionals	407
# Intergenic dREG bidirectionals	207
Part of Tfit master merge?	True
Part of dREG master merge?	True