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SRR8669176 Sample ID 2554
| Paper identifier | Vihervaara2021stress |
| SRP accession | SRP187541 |
| GEO accession | GSE127844 |
| Samples | SRR8669176 |
| QC score | 1 |
| Replicate | 1 |
| Protocol | PRO-seq |
| Library prep | ligation |
| Spike-in | D. melanogaster |
| Single/Paired end | single |
| Raw strandedness | reverse |
| Reverse complemented? | True |
| Mapped strandedness | forward |
| Timecourse | True |
| Control/Experimental | experimental |
| Wildtype & Untreated? | False |
| Outlier | No |
| Unusable | No |
| TDF visualization file | TDF file | 149 Mb |
| Stranded gene read counts | TXT file | 1 Mb |
| Unstranded bidirectional read counts | TXT file | 21 Mb |
| Tfit bidirectional regions | BED file | 3 Mb |
| dREG bidirectional regions | BED file | 1 Mb |
| All data files | ZIP archive | |
| Organism | H. sapiens |
| Sample type | cell line |
| Cell type | K562 |
| Clone/Individual | |
| Strain | |
| Genotype | |
| Construct | shRNA Hsf1 |
| Condition type | Treatment | Start time | End time | Duration |
| treatment | heat shock (42 Celsius) | -30 min | 0 min | 30 min |
| treatment | heat shock (42 Celsius) | -2970 min | -2910 min | 1 hr |
| treatment | heat shock (42 Celsius) | -3150 min | -3090 min | 1 hr |
| treatment | heat shock (42 Celsius) | -3330 min | -3270 min | 1 hr |
| treatment | heat shock (42 Celsius) | -4410 min | -4350 min | 1 hr |
| treatment | heat shock (42 Celsius) | -4590 min | -4530 min | 1 hr |
| treatment | heat shock (42 Celsius) | -4770 min | -4710 min | 1 hr |
| treatment | heat shock (42 Celsius) | -5850 min | -5790 min | 1 hr |
| treatment | heat shock (42 Celsius) | -6030 min | -5970 min | 1 hr |
| treatment | heat shock (42 Celsius) | -6210 min | -6150 min | 1 hr |
| Pipeline info and software versions | |
| Sample notes | Cites Kwak2013 for protocol and library prep and also implies ligation with adapters, but not explicitly stated |
| Analysis notes | Previously in DB until 6/29/2021 as Vihervaara2020unpublished - scripts have been changed, but all previous outerr and log files reflect previous id |
| Sample QC score | 1 |
| Sample NRO score | 2 |
| Raw read length | 76 |
| Read depth after trimming | 42,554,873 |
| Duplication proportion | 0.43098 |
| Proportion reads mapped | 0.8881 |
| Exon/intron ratio | 3.6101 |
| Unique read proportion | 0.6864 |
| Total # Tfit bidirectionals | 37574 |
| # Promoter Tfit bidirectionals | 14940 |
| # Intronic Tfit bidirectionals | 13602 |
| # Intergenic Tfit bidirectionals | 9709 |
| Total # dREG bidirectionals | 34963 |
| # Promoter dREG bidirectionals | 9313 |
| # Intronic dREG bidirectionals | 14630 |
| # Intergenic dREG bidirectionals | 10097 |
| Part of Tfit master merge? | True |
| Part of dREG master merge? | True |