DBNascent | Samples

SRR8706166 Sample ID 2142

**Data sources**
Paper identifier	Santoriello2020rna
SRP accession	SRP188036
GEO accession	GSE128081
Samples	SRR8706166

**Technical details**
QC score	2
Replicate	1
Protocol	PRO-seq
Library prep	not noted in GEO or publication
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	116 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1005 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	A375
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	A771726 (25 microM)	-24 hr	0 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	No protocol or library prep details in paper or GEO - poorly written methods
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	42
Read depth after trimming	17,945,347
Duplication proportion	0.1658
Proportion reads mapped	0.7584
Exon/intron ratio	4.36947
Unique read proportion	0.74686

**Bidirectionals**
Total # Tfit bidirectionals	34591
# Promoter Tfit bidirectionals	10961
# Intronic Tfit bidirectionals	14301
# Intergenic Tfit bidirectionals	9303
Total # dREG bidirectionals	23279
# Promoter dREG bidirectionals	9107
# Intronic dREG bidirectionals	8379
# Intergenic dREG bidirectionals	5825
Part of Tfit master merge?	True
Part of dREG master merge?	True