DBNascent | Samples

SRR8706169 Sample ID 2145

**Data sources**
Paper identifier	Santoriello2020rna
SRP accession	SRP188036
GEO accession	GSE128081
Samples	SRR8706169

**Technical details**
QC score	3
Replicate	1
Protocol	PRO-seq
Library prep	not noted in GEO or publication
Spike-in	D. melanogaster
Single/Paired end	paired
Raw strandedness	read1 reverse
Reverse complemented?	False
Mapped strandedness	read1 reverse
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	92 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	785 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	A375
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	A771726 (25 microM)	-24 hr	0 hr	1 day
treatment	nucleotides	-24 hr	0 hr	1 day

**Extended notes**
Pipeline info and software versions
Sample notes	No protocol or library prep details in paper or GEO, no details on "nucleotides" treatment - poorly written methods
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	42
Read depth after trimming	14,345,527
Duplication proportion	0.16216
Proportion reads mapped	0.7115
Exon/intron ratio	4.86927
Unique read proportion	0.74993

**Bidirectionals**
Total # Tfit bidirectionals	26834
# Promoter Tfit bidirectionals	9890
# Intronic Tfit bidirectionals	10988
# Intergenic Tfit bidirectionals	5917
Total # dREG bidirectionals	18205
# Promoter dREG bidirectionals	8090
# Intronic dREG bidirectionals	6261
# Intergenic dREG bidirectionals	3741
Part of Tfit master merge?	True
Part of dREG master merge?	True