SRR8706170 Sample ID 2146

Data sources
Paper identifier Santoriello2020rna
SRP accession SRP188036
GEO accession GSE128081
Samples SRR8706170
Technical details
QC score 2
Replicate 2
Protocol PRO-seq
Library prep not noted in GEO or publication
Spike-in D. melanogaster
Single/Paired end paired
Raw strandedness read1 reverse
Reverse complemented? False
Mapped strandedness read1 reverse
Timecourse False
Control/Experimental experimental
Wildtype & Untreated? False
Outlier No
Unusable No
Downloads
TDF visualization file TDF file
Stranded gene read counts TXT file
Unstranded bidirectional read counts TXT file
Tfit bidirectional regions BED file
dREG bidirectional regions BED file
All data files ZIP archive
Cellular details
Organism H. sapiens
Sample type cell line
Cell type A375
Clone/Individual
Strain
Genotype
Construct
Conditions and treatments
Condition type Treatment Start time End time Duration
treatment A771726 (25 microM) -24 hr 0 hr 1 day
treatment nucleotides -24 hr 0 hr 1 day
Extended notes
Pipeline info and software versions
Sample notes No protocol or library prep details in paper or GEO, no details on "nucleotides" treatment - poorly written methods
Analysis notes
Sample metrics
Sample QC score 2
Sample NRO score 3
Raw read length 42
Read depth after trimming 19,942,369
Duplication proportion 0.18802
Proportion reads mapped 0.7292
Exon/intron ratio 5.57959
Unique read proportion 0.73694
Bidirectionals
Total # Tfit bidirectionals 35924
# Promoter Tfit bidirectionals 11567
# Intronic Tfit bidirectionals 14781
# Intergenic Tfit bidirectionals 9532
Total # dREG bidirectionals 25211
# Promoter dREG bidirectionals 9063
# Intronic dREG bidirectionals 9500
# Intergenic dREG bidirectionals 6159
Part of Tfit master merge? True
Part of dREG master merge? True