DBNascent | Samples

SRR8714825 Sample ID 386

**Data sources**
Paper identifier	Boxer2020mecp2
SRP accession	SRP188217
GEO accession	GSE128184
Samples	SRR8714825

**Technical details**
QC score	2
Replicate	2
Protocol	PRO-seq
Library prep	ligation
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	True
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	82 Mb
Stranded gene read counts	TXT file	924 Kb
Unstranded bidirectional read counts	TXT file	17 Mb
Tfit bidirectional regions	BED file	3 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	M. musculus
Sample type	tissue
Cell type	forebrain
Clone/Individual
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	flavopiridol (50 mg/kg)	-40 min	0 min	40 min

**Extended notes**
Pipeline info and software versions
Sample notes	Male mice (MECP2 +/Y); vehicle or flavopiridol were injected into mice for the given time before tissue dissection
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	3
Raw read length	75
Read depth after trimming	41,137,392
Duplication proportion	0.55114
Proportion reads mapped	0.6356
Exon/intron ratio	5.89978
Unique read proportion	0.56754

**Bidirectionals**
Total # Tfit bidirectionals	32039
# Promoter Tfit bidirectionals	12315
# Intronic Tfit bidirectionals	12051
# Intergenic Tfit bidirectionals	4930
Total # dREG bidirectionals	26062
# Promoter dREG bidirectionals	8889
# Intronic dREG bidirectionals	10538
# Intergenic dREG bidirectionals	3708
Part of Tfit master merge?	True
Part of dREG master merge?	True