DBNascent | Samples

SRR8743263 Sample ID 242

**Data sources**
Paper identifier	Bi2020enhancer
SRP accession	SRP188708
GEO accession	GSE128452
Samples	SRR8743263

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	139 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	863 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual	tamoxifen-sensitive
Strain
Genotype	dox-inducible Jun (stable); shRNA Gata3 (stable)
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	doxycycline (1 microg/mL)	-2 day	0 day	2 day

**Extended notes**
Pipeline info and software versions
Sample notes	One characteristic in GEO says that these are TamR MCF7s, but everything else indicates that they're parent
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	46,906,269
Duplication proportion	0.52955
Proportion reads mapped	0.8979
Exon/intron ratio	3.22131
Unique read proportion	0.66521

**Bidirectionals**
Total # Tfit bidirectionals	26507
# Promoter Tfit bidirectionals	8842
# Intronic Tfit bidirectionals	11671
# Intergenic Tfit bidirectionals	5085
Total # dREG bidirectionals	19943
# Promoter dREG bidirectionals	7730
# Intronic dREG bidirectionals	7842
# Intergenic dREG bidirectionals	4121
Part of Tfit master merge?	True
Part of dREG master merge?	True