DBNascent | Samples

SRR8743264 Sample ID 243

**Data sources**
Paper identifier	Bi2020enhancer
SRP accession	SRP188708
GEO accession	GSE128452
Samples	SRR8743264

**Technical details**
QC score	2
Replicate	1
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	control
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	120 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	2 Mb
dREG bidirectional regions	BED file	770 Kb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	MCF7
Clone/Individual	tamoxifen-resistant
Strain
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
no treatment		None	None	None

**Extended notes**
Pipeline info and software versions
Sample notes	Characteristics in GEO are confusing, but label indicates it's another replicate of MCF7 tamoxifen-resistant cells, perhaps without 100 nM 4-OHT in culture?
Analysis notes

**Sample metrics**
Sample QC score	2
Sample NRO score	2
Raw read length	51
Read depth after trimming	48,059,018
Duplication proportion	0.61756
Proportion reads mapped	0.9206
Exon/intron ratio	3.17644
Unique read proportion	0.62925

**Bidirectionals**
Total # Tfit bidirectionals	25295
# Promoter Tfit bidirectionals	10632
# Intronic Tfit bidirectionals	10660
# Intergenic Tfit bidirectionals	3236
Total # dREG bidirectionals	17727
# Promoter dREG bidirectionals	7532
# Intronic dREG bidirectionals	6846
# Intergenic dREG bidirectionals	2964
Part of Tfit master merge?	True
Part of dREG master merge?	True