DBNascent | Samples

SRR8767376 Sample ID 1708

**Data sources**
Paper identifier	Liu2020immediate
SRP accession	SRP189113
GEO accession	GSE128698
Samples	SRR8767376

**Technical details**
QC score	4
Replicate	2
Protocol	GRO-seq
Library prep	circularization
Spike-in	None
Single/Paired end	single
Raw strandedness	forward
Reverse complemented?	False
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	12 Mb
Stranded gene read counts	TXT file
Unstranded bidirectional read counts	TXT file
Tfit bidirectional regions	BED file
dREG bidirectional regions	BED file
All data files	ZIP archive

**Cellular details**
Organism	A. thaliana
Sample type	tissue
Cell type	leaf
Clone/Individual
Strain	Col-0
Genotype
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	heat pretreatment (37 Celsius)	-10080 min	-9990 min	90 min
treatment	heat pretreatment (37 Celsius)	-1440 min	-1350 min	90 min
treatment	heat pretreatment (37 Celsius)	-2880 min	-2790 min	90 min
treatment	heat pretreatment (37 Celsius)	-4320 min	-4230 min	90 min
treatment	heat pretreatment (37 Celsius)	-5760 min	-5670 min	90 min
treatment	heat pretreatment (37 Celsius)	-7200 min	-7110 min	90 min
treatment	heat pretreatment (37 Celsius)	-8640 min	-8550 min	90 min
treatment	heat shock (45 Celsius)	-5 min	0 min	5 min

**Extended notes**
Pipeline info and software versions
Sample notes	1.5 hr heat priming treatment for a week
Analysis notes

**Sample metrics**
Sample QC score	4
Sample NRO score	1
Raw read length	30
Read depth after trimming	55,018,374
Duplication proportion	0.9388
Proportion reads mapped	0.4917
Exon/intron ratio	1.43042
Unique read proportion	0.12093

**Bidirectionals**
Total # Tfit bidirectionals	None
# Promoter Tfit bidirectionals	None
# Intronic Tfit bidirectionals	None
# Intergenic Tfit bidirectionals	None
Total # dREG bidirectionals	None
# Promoter dREG bidirectionals	None
# Intronic dREG bidirectionals	None
# Intergenic dREG bidirectionals	None
Part of Tfit master merge?	False
Part of dREG master merge?	False