DBNascent | Samples

SRR8867625 Sample ID 2318

**Data sources**
Paper identifier	Steinparzer2019transcriptional
SRP accession	SRP191474
GEO accession	GSE129501
Samples	SRR8867625

**Technical details**
QC score	3
Replicate	2
Protocol	PRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	217 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	1 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype	Cdk8 F97G
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	IFN-gamma (10 ng/mL)	-45 min	0 min	45 min
vehicle	DMSO	-45 min	0 min	45 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	76
Read depth after trimming	121,035,164
Duplication proportion	0.7037
Proportion reads mapped	0.9371
Exon/intron ratio	3.95222
Unique read proportion	0.45599

**Bidirectionals**
Total # Tfit bidirectionals	43030
# Promoter Tfit bidirectionals	19032
# Intronic Tfit bidirectionals	13793
# Intergenic Tfit bidirectionals	10007
Total # dREG bidirectionals	33027
# Promoter dREG bidirectionals	8852
# Intronic dREG bidirectionals	12594
# Intergenic dREG bidirectionals	10073
Part of Tfit master merge?	True
Part of dREG master merge?	True