DBNascent | Samples

SRR8867627 Sample ID 2320

**Data sources**
Paper identifier	Steinparzer2019transcriptional
SRP accession	SRP191474
GEO accession	GSE129501
Samples	SRR8867627

**Technical details**
QC score	3
Replicate	2
Protocol	PRO-seq
Library prep	not noted in GEO or publication
Spike-in	None
Single/Paired end	single
Raw strandedness	reverse
Reverse complemented?	True
Mapped strandedness	forward
Timecourse	False
Control/Experimental	experimental
Wildtype & Untreated?	False
Outlier	No
Unusable	No

**Downloads**
TDF visualization file	TDF file	230 Mb
Stranded gene read counts	TXT file	1 Mb
Unstranded bidirectional read counts	TXT file	21 Mb
Tfit bidirectional regions	BED file	4 Mb
dREG bidirectional regions	BED file	2 Mb
All data files	ZIP archive

**Cellular details**
Organism	H. sapiens
Sample type	cell line
Cell type	HCT116
Clone/Individual
Strain
Genotype	Cdk8 F97G
Construct

**Conditions and treatments**
Condition type	Treatment	Start time	End time	Duration
treatment	3MB-PP1 (10 microM)	-45 min	0 min	45 min
treatment	IFN-gamma (10 ng/mL)	-45 min	0 min	45 min

**Extended notes**
Pipeline info and software versions
Sample notes
Analysis notes

**Sample metrics**
Sample QC score	3
Sample NRO score	2
Raw read length	76
Read depth after trimming	128,996,393
Duplication proportion	0.71056
Proportion reads mapped	0.94
Exon/intron ratio	3.85807
Unique read proportion	0.47339

**Bidirectionals**
Total # Tfit bidirectionals	46694
# Promoter Tfit bidirectionals	19809
# Intronic Tfit bidirectionals	15280
# Intergenic Tfit bidirectionals	11792
Total # dREG bidirectionals	38607
# Promoter dREG bidirectionals	9441
# Intronic dREG bidirectionals	15107
# Intergenic dREG bidirectionals	12650
Part of Tfit master merge?	True
Part of dREG master merge?	True